Biochemical study of the relationship of extracellular glucan to adherence and cariogenicity in streptococcus mutans and an extracellular polysaccharide mutant. Effect of nicotine on dualspecies biofilms of streptococcus. Streptococcus mutans is known as a primary pathogen responsible for dental caries. Role of streptococcus mutans in human dental decay.
Serotype distribution of streptococcus mutans a pathogen of. Characterization of recombinant, ureolytic streptococcus mutans demonstrates an inverse relationship between dental plaque ureolytic capacity and cariogenicity k. These multiple interactions are based on the biology of the streptococci as well as on the physical effects of previous attachments, and the colonization regulates bio. Cariogenicity of streptococcus mutans strain with defects in fructan metabolism assessed in a programfed specific pathogenfree rat model. Streptococcus mutans is involved in biofilm formation. Center for immunology and microbial disease,albany medical college. The colonies of strains grown on mitis salivarius agar were similar in morphology to those of mutans like streptococci. Biology, immunology, and cariogenicity of streptococcus. However, further analysis revealed that transcription of the ftf gene was initiated at the p1 promoter contained. Here we report the complete genome sequence of serotype k s. Free biology and life science resources at your fingertips. Antibacterial effects of natural herbal extracts on. Serotype k, recently designated and initially found in blood isolates, features a drastic.
Molecular and genetic analysis of multiple changes in the. The aim of the study was to establish a relationship of dental caries with the gtf and streptococcus mutans count to determine the caries active individuals. The level of caries increased significantly p less than or equal to 0. Detection of cariogenic streptococcus mutans in extirpated. Serotype distribution of streptococcus mutans a pathogen. Biology of streptococcus mutansderived glucosyltransferases. Streptococcus mutans, an assessment of its physiological potential in relation to dental caries. Since they bear genetic similarities and are large in size, differentiation of their. Metabolic modeling of streptococcus mutans reveals complex. Chronic pulpal inflammation under caries appears to be elicited by bacterial antigens that diffuse into the pulp through dentinal tubules. Sortase a is an enzyme responsible for the covalent attachment of pac proteins to the cell wall in streptococcus mutans. Development of a pcr method for rapid identification of. Google scholar russell mw, challacombe sj, lehner t. The major cell surface protein p1, also known as antigen iii ca.
Pdf streptococcus mutans is one of cariogenic microorganisms associated with tooth decay. Streptococcus mutans is a key cariogenic bacterium responsible for the initiation of tooth decay. During screening for antiplaque agents of plant origin, ethanolic extracts from melaphis chinensis bell, the chinese nutgall, exhibited strong inhibition of glucosyltransferase gtf, in vitro adherence and glucaninduced agglutination of streptococcus mutans. Comparative inhibitory effects of 4allylpyrocatechol. The extent of adaptation was indicated by changes in minimum ph values attained by harvested cells suspended in dense. This study aims to evaluate the antimicrobial activity of garlic bulbs, pudina leaves, and mango and eucalyptus twig extracts on streptococcus mutans by evaluating their zone of inhibition and determining their minimum inhibitory concentration mic. A blood isolate of streptococcus mutans strain tw871 shows relatively low homology with mt8148, a reference oral isolate strain, and lacks the serotypespecific polysaccharide antigen, suggesting that other cellsurface structures correlate with cariogenicity. The quorumsensing system of streptococcus mutans is mediated by the competencestimulating peptide csp, whose precursor is encoded by the comc gene. Frontiers streptococcus mutansderived extracellular matrix. To better understand the regulation of the expression of these genes. However, no information is available regarding the properties of s. Serum glucosyltransferaseinhibiting antibodies and dental caries in rhesus monkeys immunized against streptococcus mutans.
Bacterial components associated with the adhesion phase of s. Intrafamilial distribution of mutans streptococci in japanese. We compared cariogenicity of tw871 with mt8148 serotype c and blood isolate tw964. Cariogenicity of streptococcus mutans v403 glucosyltransferase and fructosyltransferase mutants constructed by allelic exchange. Curcumin, an active ingredient of turmeric, was reported to be an inhibitor for staphylococcus aureus sortase. They produce a homologous exocellular polysaccharide called glucan, which strongly adheres to the enamel surface. Sequence analysis of the mutant revealed a mutation in gida, which is known to be involved in trna modification in streptococcus pyogenes. Multiple streptococcus mutans genes are involved in biofilm. Streptococcus intermedius, streptococcus mutans, and candida albicans are harmful oral pathogens and prone to resist chemical antimicrobial agents. Streptococcus mutans is generally considered to be the principal aetiological agent. Glucan plays a central role in sucrosedependent biofilm formation by the dental pathogen streptococcus mutans.
Genotypic diversity of streptococcus mutans in cariesfree and. Department of oral biology, school of dentistry, university of colorado health sciences center, denver, colorado 80262. The main virulence factors associated with cariogenicity include adhesion, acidogenicity, and acid tolerance. This prompted the hypothesis that cytokines elicited by antigens from streptococcus mutans, which frequently dominates shallow lesions, could play a major role in eliciting the initial tcell response in the pulp. The involvement of oral bacteria in the pathogenesis of cardiovascular disease has been studied, with streptococcus mutans, a pathogen of dental caries, detected in cardiovascular lesions at a high frequency. This cellular adhesion plays an important role in the formation of dental plaque and the initiation of dental caries. Streptococcus mutans is the major pathogen of dental caries and occasionally causes infective endocarditis. Adaptation of streptococcus mutans and enterococcus hirae. Within the complex oral microbiome, streptococcus mutans is a major producer of extracellular polymeric substances including exopolysaccharides eps, edna, and lipoteichoic acid lta. Streptococcus mutans is the major pathogenic agent causing dental caries 3, 5, 6. In vitro efficacy assessment of some toothpastes and miswak. Jun 01, 2012 read tea catechin epigallocatechin gallate inhibits streptococcus mutans biofilm formation by suppressing gtf genes, archives of oral biology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Asm journals are the most prominent publications in the field, delivering uptodate and authoritative coverage of both basic and clinical microbiology.
The effect of carbohydrate restriction on the presence of streptococcus mutans, streptococcus sanguis and iodophilic polysaccharideproducing bacteria in human dental plaque. Allelic replacement of the c terminus of a streptococcus mutans surface protein affects murein hydrolase activity. The importance of streptococcus mutans in the etiology and. Streptococcus mutans has been strongly implicated as the principal etiological agent in dental caries. Streptococcus mutans produces a number of extracellular sucrosemetabolizing enzymes that contribute to the ability of the organism to cause dental caries, including three glucosyltransferases, the products of the gtfb, gtfc and gtfd genes, and a fructosyltransferase, encoded by the ftf gene. Dental caries is one of the worlds most major and prevalent diseases affecting 6090% of school children and most of the adult population petersen et al. Gnotobiotic rats infected with streptococcus mutant 6715, mutans c211 at 45 days of age on provided a purified diet containing 5% sucrose developed carious lesions on buccal, sulcal, and proximal molar surfaces within 15 days 60 days of age. Burne department of microbiology and immunology and center for oral biology. Biology, immunology and cariogenicity of streptococcus mutans. Tea catechin epigallocatechin gallate inhibits streptococcus. Streptococcus mutans has been implicated as a primary causative agent of dental caries in humans. Development of a pcr method for rapid identification of new.
Regulation of the gtfbc and ftf genes of streptococcus. The full text of this article hosted at is unavailable due to technical difficulties. Roles of sortase in surface expression of the major. Streptococcus mutans is a gram positive bacterium that thrives under acidic conditions and is a primary cause of tooth decay dental caries.
A novel gene involved in the survival of streptococcus mutans. Streptococcus mutans society for applied microbiology. Changes in the expression of metabolic proteins were generally limited to three biochemical. Twodimensional gel electrophoretic analysis of the proteome of streptococcus mutans grown at a steady state in a glucoselimited anaerobic continuous culture revealed a number of proteins that were differentially expressed when the growth ph was lowered from ph 70 to ph 50. Nagra av dessa andra bakterier ar streptococcus sanguis, s. Attenuation of glucanbinding protein c reduces the. Three strains of streptococcus mutans belonging to serotypes a, c, and f were transformed to streptomycin resistance by deoxyribonucleic acids derived from homologous and heterologous streptomycinresistant strains of s. Active ingredients from plants are of increasing interest as an alternative. The importance of streptococcus mutans in the etiology and pathogenesis of dental caries is certainly controversial, in part because excessive attention is paid to the numbers of s. A fructosyltransferase ftf gene from streptococcus mutans gs5 has been isolated from a xl47. Inhibitory effect of surface prereacted glassionomer sprg.
Virulence factors of streptococcus mutans and dental. Streptococcus mutans produces several enzymes which metabolize sucrose. The unidentified 65kda cell surface protein sp of s. Virtual special issue on apoptosis and viral infection. Here we demonstrated that clpp was involved in biofilm formation of s. Pdf virulence properties of streptococcus mutans researchgate. Gallotannins inhibit growth, waterinsoluble glucan. In vitro efficacy assessment of some toothpastes and. Curcumin reduces streptococcus mutans biofilm formation by.
The main virulence factors associated with cariogenicity. Streptococcus mutans, a pathogen of dental caries, is known to be associated with bacteremia and infective endocarditis ie. Pdf streptococcus mutans is considered one of the primary causative agents of. Pdf virulence factors of streptococcus mutans and dental.
This study investigated the effect of nicotine on mono and dualspecies growth of s. Differential and quantitative analyses of mrna expression of. Characterization of recombinant, ureolytic streptococcus. Differential and quantitative analyses of mrna expression. Streptococcus mutans and cardiovascular diseases researchgate. Streptococcus mutans gs5 and ib1600 adapted to growth in acidic environments in continuous culture at slow generation time 8. This study aims to compare antimicrobial effects of 4allylpyrocatechol apc extracted from piper betle on these oral pathogens. Role in extracellular matrix formation of cariogenic biofilms. Seventythree strains of streptococci were isolated from the bat oral cavity.
Several surface proteins have been shown to be involved in this process. Streptococcus mutans, which is known to be a major cariogenic bacterium as well as one of the pathogens involved with bacteremia and infective endocarditis ie, was previously classified into three serotypes, c, e, and f, based on the chemical composition of serotypespecific polysaccharides. Biofilms are highly structured microbial communities that are enmeshed in a selfproduced extracellular matrix. A mutant of streptococcus mutans, gs5, which differed in extracellular polysaccharide eps produced from sucrose, was used to study the role of eps in the production of dental caries.
Mar 22, 2018 streptococcus mutans has been implicated as a primary causative agent of dental caries in humans 1. Although the mechanisms of dental caries have been well investigated and the incidence of dental. Effects of host immune responses on the establishment and pathogenesis. Interaction of glucosyltransferase from streptococcus mutans with various glucans. Clpp affects biofilm formation of streptococcus mutans. Get a printable copy pdf file of the complete article 10m, or click on a page image below. Full text full text is available as a scanned copy of the original print version. The mutant proved to be identical to the parent strain in sugar fermentation, growth rate, and serotype. Streptococcus mutans produces three glucosyltransferases, coded by gtfb, gtfc, and gtfd, whose cooperative action is essential for sucrosedependent cellular adhesion.
Streptococcus mutans definition and examples biology online. Biology, immunology, and cariogenicity of streptococcus mutans. The targeted open reading frame encodes a 67kda protein smaa with an nterminal signal sequence and cleavage site, three 46aminoacid aa direct repeats, and two 88aa direct repeats. Heart valve specimens were collected from 52 patients and. A comc mutant of strain gs5 exhibited enhanced antimicrobial sensitivity to a wide variety of different agents. Read typing of mutans streptococci by arbitrarily primed polymerase chain reaction, archives of oral biology on deepdyve, the largest online rental service for scholarly research with thousands of academic publications available at your fingertips. Bacteria utilize quorumsensing systems to modulate environmental stress responses.
Both streptococcus mutans and streptococcus sanguinis are normal bacterial inhabitants of dental plaque. Streptococcus mutans is considered one of the primary causative agents of dental caries and can also be a source of infective endocarditis. Complete genome sequence of the serotype k streptococcus. Regulation of the gtfbc and ftf genes of streptococcus mutans. Streptococcus mutans murein hydrolase journal of bacteriology. The abilities to form biofilms on teeth surface and to metabolize a wide range of carbohydrates are key virulence attributes of streptococcus mutans. It has been well documented that nicotine affects the growth of s. The serotypespecific polysaccharide was shown to be composed of. One of the important virulence properties of these organisms is their ability to form biofilms known as dental plaque on tooth surfaces. Biochemical study of the relationship of extracellular glucan to adherence and cariogenicity in streptococcus mutans and an extracellular. It has been shown to play a role in modulating the surface properties and the biofilm formation and influence the cariogenicity of s.
Find the definition of streptococcus mutans in the largest biology dictionary online. New serotype of mutans streptococci isolated from pig oral. The analysis of 1,908 isolates cultured from the dental plaque of 76 subjects from 20 families 20 married couples and 36 of their children resulted in the identification of 144 genotypes containing 114 strains of streptococcus mutans serotype c, 66. Streptococcus mutans is a pioneer bacteria implicated in dental caries. The role of glucanbinding proteins in the cariogenicity. Biofilm formation by streptococcus mutans and related bacteria. Clpp inactivation resulted in enhanced biofilm formation or initial cell. Clpp has been proved to play an important role in biofilm development in streptococci. Effect of specific antisera on adherence properties of the. A streptococcus mutans mutant defective in aciduricity was constructed by randominsertion mutagenesis.
Streptococcus mutans was frequently detected in the heart valve 69% and atheromatous plaque 74% specimens, while other bacterial species, including those related to periodontitis, were detected with much lower frequencies. Streptococcus mutans and cardiovascular diseases sciencedirect. Biochemical study of the relationship of extracellular. A 29 kda surface protein named wallassociated protein a wapa, antigen a or antigen iii, was. C munro, s m michalek, and f l macrina department of microbiology and immunology, virginia commonwealth university, richmond 232980678. Proteome analysis of streptococcus mutans metabolic. Three glucosyltransferase genes gtfb, gtfc, and gtfd and a single fructosyltransferase gene ftf encode enzymes which are important in formation of exopolysaccharides.
Cytokine induction by streptococcus mutans and pulpal. To better understand the regulation of the expression of these genes under environmental conditions. Glycosyltransferasemediated biofilm matrix dynamics and. Streptococcus mutans possesses the abilities to adhere to pelliclecoated tooth surfaces and to form acids two characteristics associated with the cariogenicity of this microorganism. The same sera were used to study the involvement of these antigens in cellcell aggregation mediated by sucrose or water. Role of bacteriocin immunity proteins in the antimicrobial. Since the roles of sucrose and glucosyltransferases in s.
Glucanbinding domain of a glucosyltransferase from. Typing of mutans streptococci by arbitrarily primed. Biology, immunology, and cariogenicity of streptococcus mutanst. Isolation and characterization of a fructosyltransferase. Streptococcus mutans, a pathogen of dental caries, is known to be associated with bacteremia and. Virulence factors of streptococcus mutans and dental caries. Furthermore, broadrange pcr with dna sequencing analysis was employed to identify the bacterial species in those samples. Streptococcus mutans is the major agent causing dental caries.
The bacterium has been classified into four serotypes, c, e, f, and k, based on the chemical composition of the serotypespecific rhamnoseglucose polymers. Slade, biology, immunology, and cariogenicity of streptococcus mutans. Streptococcus mutans is a facultatively anaerobic, grampositive coccus round bacterium commonly found in the human oral cavity and is a significant contributor to tooth decay. Center for oral biology and department of microbiology and immunology, university of rochester.
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